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Processing of undifferentiated 3T3-L1 pre-adipocytes (day 0) and differentiated adipocytes (day 10) were optimized for recovery and reproducibility of 1-MNA levels across cultured batches of 3T3-L1 cells (˜passages 7-8) and the 1-MNA levels were compared between the pre-adipocytes and adipocytes

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The mandible and the gingiva around the bottom molars were excised