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For the coimmunoprecipitation (co-IP) assay, cells were harvested and then lysed with IP buffer (20 mM Tris-HCl [pH 7.4], 150 mM NaCl, 10% glycerol, 2 mM EDTA, 0.5% Nonidet P-40, 0.5% Triton X-100) with 1% protease and phosphatase inhibitor (Roche)

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Why Hollywood Celebrities Take Glutathione Here’s what we’ll cover in this blog post: What is Glutathione Why celebrities take Glutathione Benefits of Glutathione A growing number of celebrities are taking Glutathione in their quest for longevity and a youthful appearance