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Retatrutide Reconstitution Retatrutide reconstitution math Vial Size 5 mg BAC Water 1 mL Concentration 5 mg/mL 2 mg dose 0.4 mL (40 U) 4 mg dose 0.8 mL (80 U) 8 mg dose — 12 mg dose — Vial Size 10 mg BAC Water 2 mL Concentration 5 mg/mL 2 mg dose 0.4 mL (40 U) 4 mg dose 0.8 mL (80 U) 8 mg dose 1.6 mL (160 U)* 12 mg dose — Vial Size 10 mg BAC Water 1 mL Concentration 10 mg/mL 2 mg dose 0.2 mL (20 U) 4 mg dose 0.4 mL (40 U) 8 mg dose 0.8 mL (80 U) 12 mg dose 1.2 mL (120 U)* Vial Size 20 mg BAC Water 2 mL Concentration 10 mg/mL 2 mg dose 0.2 mL (20 U) 4 mg dose 0.4 mL (40 U) 8 mg dose 0.8 mL (80 U) 12 mg dose 1.2 mL (120 U)* *Volumes over 1.0 mL need a larger syringe or a split injection at two sites

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The pLVX-TetOne-Puro vector was a gift from Rongqing Li (Xuzhou Medical University, Xuzhou, China)