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Furthermore, according to the in vivo data obtained by single–voxel proton magnetic resonance spectroscopy (1H-MRS), GABA levels are also reduced in the PWS brain (at least, in the parieto-occipital lobe) [121], hinting at the possibility of existence of a feedback loop regulating either GAD1 expression or GAD67 enzymatic activity in GABAergic neurons in response to the fluctuations of GABA A Rs’ content in PyCs