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In contrast to the early events, phase-contrast microscopy of the 24-h R001-treated TNBC cells in culture showed altered cellular morphology indicative of fluid-filled cells due to plasma membrane leakage (Fig
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Fluorescent intensities were recorded from the lysates in a spectrofluorometer (SpectraMax M3, Molecular Devices ) at 502 nm and 523 nm excitation and emission wavelengths, respectively
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