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Immunopeptidomics HLA peptide isolation and purification At the time of harvest, tissue culture cells were washed with 1X PBS, removed from plates by using 0.05% Trypsin-EDTA, recovered by centrifugation at 1000 g for 3 min, washed once with 1X PBS, and snap frozen in liquid N 2

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Your nutrient infusion should take less than an hour, and you may even feel the results before the treatment is over