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Tian and colleagues extended this with an ultra-high-performance liquid chromatography high-resolution mass spectrometry workflow using 13C and 15N labelled peptide for nontargeted metabolite identification, reporting detection limits of 0.01 to 0.11 nanograms per milliliter, linearity across 0.02 to 50 nanograms per milliliter at an r-squared above 0.999, and recovery above 90 percent
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