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Briefly, HepG2 cells were seeded on a 6-well plate (2 10 5 cells/well) and treated with 10 μM of SF or control (DMSO) in serum-free DMEM without glucose (Gibco #:11966025) supplemented with 1 mM sodium pyruvate (Gibco #:11360070), and 5.5 mM or 25 mM glucose (Gibco #: A2494001)
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