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After 24 h, the cells were transfected with 2 µg of the lentiviral Gag-Pol expression construct pCMV-ΔR8.74 72 , 2 µg of the reporter plasmid coding for a firefly luciferase (pWPI-F-Luc-BLR) 73,74 and 2 µg of either pcDNA3_CMV_dcE1E2_Con1, pcDNA3_CMV_dcE1E2_JFH 75 or pczVSV-G 76 coding for the glycoproteins of interest or 2 µg of the empty vector control (pcDNA3)

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Thymulin May be suggested for immune modulation and inflammation balance