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Carbon-coated copper EM grids (400 mesh Cu-film, Electron Microscopy Sciences, Hatfield, PA) were glow-discharged in a Solarus plasma cleaner (Gatan, Pleasanton, CA) for 20 s at 10 W in air and subsequently were held using reverse anti-capillary tweezers, carbon side up, and a 4 μl drop of the diluted ArqI sample was applied at RT and after 1 min blotted with Whatman 1 filter paper
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