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(c) transcellular active transport used by nutrients as sugars, amino acids and vitamins that require specific transporters and energy and (d) endocytosis and basolateral exocytosis via vesicles are used by larger peptides and proteins, large bacterial components or even whole bacteria ( in vivo studies assessing human intestinal permeability (oral administration of sugars or other probes later detected in blood or urine) mainly target the paracellular pathway