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HEK 293T cells (3 × 10 5 cells per 2 ml DMEM) were cultured for 24 h and then transfected with pcDNA4/TO or pcFLAG-h Mgst2 (1 μg ml −1 ) using jetPEI (2 μg ml −1 , Polyplus-transfection) as a transfection reagent

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