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The butyryl group-preference of Sirt6 is consistent with the previous kinetic analysis, in which catalytic efficiency ( k cat / K m ) of Sirt6 on H3K9-butyrylated peptides (10 s −1 M −1 ) was about twice as much as that on H3K9-acetylared peptides (4.8 s −1 M −1 ) 40