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In contrast, the 2,3-diamino substitution in the 1MQ scaffold (2,3-diamino-1MQ) displayed high passive permeability (Table 4), but moderate bi-directional active transport with moderate efflux ratio (Table 5)
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RBDs from Bat CoV RaTG13, Bat CoV SHC014, Bat CoV Rs4081, Bat CoV RmYN02, Bat CoV Rf1, Bat CoV WIV1, Pangolin CoV Pang17, and SARS-CoV-2 were expressed on SpyTag proteins, which were then fused to one SpyCatcher protein with multiple domains, leading to the generation of the mosaic-8b nanoparticle vaccine [141]
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