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Although alterations in Bax, Bcl-2, and caspase-3 expression strongly indicate activation of the intrinsic apoptotic pathway, mitochondrial integrity or membrane potential (ΔΨm) was not directly assessed in this study

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This strategy offers a noninvasive biomarker for early screening, validated by Tang et al.'s work in BTBR mice, which revealed a decrease in parvalbumin-positive interneurons and glutamate/GABA imbalances in the circuits of the auditory cortex [108]